Work

You can find below a selection of relevant publications grouped by main research topic. Here you can find the complete list, and here you can find it as a Google Scholar entry. This is my ORCID id: 0000-0002-4964-3264.

Biomarkers analysis

I’m interested in exploring the optimal way to detect biomarkers from high throughput screenings. The work in collabortion with the protein array lab at INGM is focusing on leveraging the power of combinations of sub-optimal biomarkers. 

• M. Bombaci, R.L. Rossi c Computation and Selection of Optimal Biomarker Combinations by Integrative ROC Analysis Using CombiROC. In: Brun V., Couté Y. (eds) Proteomics for Biomarker Discovery. Methods in Molecular Biology (2019), vol 1959. Humana Press, New York, NY
• S. Mazzara =, R.L. Rossi =,c , R. Grifantini, S. Donizetti, S. Abrignani, M. Bombaci. CombiROC: an interactive web tool for selecting accurate marker combinations of omics data. Scientific Reports (2017) 7, 45477.
  • See the web application: CombiROC, guided and interactive generation of multimarker panels
• S. Mazzara, A. Sinisi, A. Cardaci, R.L. Rossi, L. Muratori, S. Abrignani, M. Bombaci . Two of Them Do It Better: Novel Serum Biomarkers Improve Autoimmune Hepatitis Diagnosis. PLoS One (2015); 10(9): e0137927.

Neuroscience of Rett Syndrome

Recent work on Rett Syndrome mouse models with dr. Bedogni from OSR is allowing us to uncover molecular determinants of this disease in the light of neurological development.

• C. Cobolli Gigli, L. Scaramuzza, M. De Simone, R.L. Rossi, D. Pozzi, M. Pagani, N. Landsberger, F. Bedogni. Lack of Methyl-CpG Binding Protein 2 (MeCP2) Affects Cell Fate Refinement During Embryonic Cortical Development. Cerebral Cortex. (2018) 28(5):1846-1856.
• F. Bedogni, C. Cobolli Gigli, D. Pozzi, R.L. Rossi, L. Scaramuzza, G. Rossetti, M. Pagani, C. Kilstrup-Nielsen, M. Matteoli, N. Landsberger. Defects During Mecp2 Null Embryonic Cortex Development Precede the Onset of Overt Neurological Symptoms. Cerebral Cortex (2016); 26(6): 2517-29, Epub May2015.
• F. Bedogni, R.L. Rossi, F. Galli, C. Cobolli Gigli, A. Gandaglia, C. Kilstrup-Nielsen, N. Landsberger. Rett syndrome and the urge of novel approaches to study MeCP2 functions and mechanisms of action. Neurosci Biobehav Rev. (2014); 46(2):187-201.

Non coding RNAs

I’ve been thoroughly involved in non coding RNA – expecially microRNAs – research in human cells, both as molecular regulation determinants and as circulating serum biomarkers. Some of our investigations led to the development of ad-hoc analytical tools alleviating issues of microRNAs normalization and not consistent nomenclature.

• A. Cherubini, M. Barilani, R.L. Rossi, M.M.K. Jalal, F. Rusconi, G. Buono, E. Ragni, G. Cantarella, H.A.R.W. Simpson, B. Péault, L. Lazzari. FOXP1 circular RNA sustains mesenchymal stem cell identity via microRNA inhibition. Nucleic Acids Res (2019); 47(10): 5325-5340.
• A Torri, D Carpi, E Bulgheroni, MC Crosti, M Moro, P Gruarin, R.L. Rossi, G. Rossetti, D. Di Vizio, M. Hoxha, V. Bollati, C. Gagliani, C. Tacchetti, M. Paroni, J. Geginat, L. Corti, L. Venegoni, E. Berti, M. Pagani, G. Matarese, S. Abrignani, P. de Candia. Extracellular microRNA signature of human helper T cell subsets in health and autoimmunity. Journal of Biological Chemistry (2017); 292 (7): 2903-15.
• F. Marabita, P. de Candia, A. Torri, J. Tegnér, S. Abrignani, R.L. Rossi c. Normalization of circulating microRNA expression data obtained by quantitative real-time RT-PCR. Briefings in Bioinformatics (2016); 17(2): 2014-12.
• R.J.P. Bonnal =, R.L. Rossi = , D. Carpi, V. Ranzani, S. Abrignani, M. Pagani. miRiadne: a web tool for consistent integration of miRNA nomenclature. Nucleic Acids Research (2015) 43(W1):W487-W492.
  • See the web application: Miriadne: a tool for re-annotating miRNA namelists or datasets.
• M. Pagani, G. Rossetti, I. Panzeri, P. de Candia, R.J.P. Bonnal, R.L. Rossi, J. Geginat, S. Abrignani. Role of microRNAs and Long-non-coding RNAs in CD4+ T cell differentiation. Immunological Reviews. (2013), 253(1):82-96.
• R.L. Rossi = , G. Rossetti =, L. Wenandy =, M. Moro, R. Sciarretta Birolo, R.J.P. Bonnal, S. Curti, S. Maglie, P. Gruarin, F. Marabita, D. Mascheroni, A. Ripamonti, M. Comelli, E. Trabucchi, R. De Francesco, J. Geginat, S. Abrignani and M. Pagani. Distinct microRNA signatures in human lymphocyte subsets and enforcement of the naïve state in CD4+ T cells by the microRNA miR-125b. Nature Immunology (2011),12(8):796-803.

Others

In my work as a staff scientist I’m exposed to other issues at the interface between wet bench lab research and computational biology/bioinformatics. Below a few examples touching big data, hematology, epigenetics and immunology.

• R.L.Rossi c & R. Grifantini c. Big data: challenge and opportunity for translational and industrial research. Frontiers in Digital Humanities (2018) 5:13.
• G. Gritti, A. Gianatti, F. Petronzelli, R. DeSantis, C. Pavoni, R.L. Rossi, L. Cattaneo, L.G. Spagnoli, S. Ferrari, A. Rossi, A.M. Barbui, A. Rambaldi. Evaluation of tenascin-C by tenatumomab in T-cell non-Hodgkin lymphomas identifies a new target for radioimmunotherapy. Oncotarget (2018); 9, 9766-9775.
  PMID 29515769; DOI: 10.18632/oncotarget.23919; EID: 2-s2.0-85041700291
• L. Fagnocchi, A. Cherubini, H. Hatsuda, A. Fasciani, S. Mazzoleni, V. Poli, V. Berno, R. L. Rossi, R. Reinbold, M. Endele, T. Schroeder, M. Rocchigiani, Z. Szkarłat, S. Oliviero, S. Dalton, A. Zippo. A Myc-driven self-reinforcing regulatory network maintains mouse embryonic stem cell identity. Nature Communication 7, 11903 (2016).
  PMID 27301576; DOI: 10.1038/ncomms11903; EID: 2-s2.0-84975038181; WOS:000378685300001
• C. Faliti, R. Gualtierotti, E. Rottoli, M. Gerosa, L. Perruzza, A. Romagnani, G. Pellegrini, B. De Ponte Conti, R.L. Rossi, M. Idzko, E. Mazza, S. Bicciato, E. Traggiai, P.L. Meroni, and F. Grassi. P2X7 receptor restrains pathogenic Tfh cells generation in systemic lupus erythematosus. J. Exp. Med. (2019) 216(2).
  PMID 30655308; DOI: 10.1084/jem.20171976; EID: 2-s2.0-85061114504